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Description
Mouse TL ELISA KitProduct Specification Sensitivity 9. 375ng ml Species Reactivity Mouse Theory This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin labeled anti TC antibody. Free components are washed away. HRP streptavidin (SABC) is
Product Specification
| Sensitivity | 9.375ng/ml | ||||||||||||||||||||||||||||||||||
| Species Reactivity | Mouse | ||||||||||||||||||||||||||||||||||
| Theory | This kit uses competitive ELISA assay, and the experimental duration is 2 hours. The plates supplied in the kit have been pre-coated with TC. The TC to be tested in the sample or standard will compete with the TC previously coated on the microplate for binding to a fixed amount of biotin-labeled anti-TC antibody. Free components are washed away. HRP-streptavidin (SABC) is added, and biotin specifically binds to streptavidin to form an immune complex. The unbound conjugate was washed off and TMB chromogenic substrate was added. The TMB became blue under the catalysis of horseradish peroxidase (HRP) and turned yellow after the reaction stop solution was added. The OD value was measured at 450nm with a microplate reader. Using curve equation software, the OD450 value of the sample was compared to the standard curve to determine the concentration of TC in the sample. There is an inverse proportion between the concentration of the target substance and the OD450 value. | ||||||||||||||||||||||||||||||||||
| Synonym | Mouse TC (Total cholesterol) ELISA Kit | ||||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | ||||||||||||||||||||||||||||||||||
| Composition |
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| General Notes | 1. When using different kits, it is necessary to mark them first to prevent the components from being mixed, resulting in experimental failure. 2. After the kit is opened, please refer to the component storage conditions table for the storage conditions of the enzyme plate and standard product (the activity will decrease after moisture). 3. Please use a sterile disposable tip to suck the reagent. After use, the reagent bottle cap must be tightened to prevent microbial contamination and evaporation. 4. When washing the plate manually, add the tip or dropper of the washing liquid and do not touch the hole of the enzyme labeled plate. Inadequate washing or contamination tend to cause false positives and high backgrounds. 5. During the testing process, please prepare the reagents required for the next experiment in advance, and add the reagents to the plate holes in time after washing the plate to prevent the plate holes from drying out and causing testing failure. 6. Do not use reagents from other batches of kits or reagents from other sources for this kit without confirmation. 7. Do not reuse disposable tips to avoid cross-contamination. 8. After the sample addition is completed, the film is applied to prevent the sample from evaporating during the incubation process, and the incubation process is completed at the recommended temperature. 9. Please wear laboratory coats, masks, gloves, etc. during the test, and do a good job of protection. Especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations. |
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| Instructions | Serum, plasma, cell culture supernatant, cell lysate and other fluid samples | ||||||||||||||||||||||||||||||||||
| Storage Temp. | Unopened kit, stored at 2-8 °C, shelf life for one year. | ||||||||||||||||||||||||||||||||||
| Test Range | 15.625-1000ng/ml |
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4.4 ★★★★★
Based on 197 reviews
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Product Reviews
★★★★★ 4
amazing
Format: Kindle
Knot the Bride was a fantastic read! The characters were all amazing and well-developed. It was easy to like them all. Sophia, Luca, Nick, and Gavin were all perfect for each other. It was such a charming story that had me hooked the entire time. I did wish there were POVs from Luca, Nick, and Gavin but it was still an amazing book without it.
I am excited to read the next book in the Willowside Omegaverse series! This is definitely a must-read for fans of omegaverse romance!.
I received a free copy of this book and am voluntarily leaving a review.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 25, 2025
★★★★★ 3
3 Star Read,
Format: Kindle
This book wasn't bad, but wasn't my cup of tea. It's highly disappointing because the storyline is so original. There is no real conflict or resolution, so the entire thing feels flat.
As a lover of omegaverse books, I know there is a ton of variety out there, and ov is really up to the author. But this one is weird. Omegas have multiple scent glands all over their bodies and go into week long heats every month. Alphas have knots in the middle of their shaft instead of the base, and the knot doesn't always swell, no explanation of when or why. It doesn't engage at all when the mouth is in play.
I also didn't enjoy the author's writing style. Each paragraph is only 1 or 2 sentences long, and the entire book reads very stacato. The conversations are stiff and unnatural feeling. Everything is very repetitive, both in word choice and in thought. The same thing is repeated 3 or 4 times over a single page, multiple times over. I ended up doing so much skimming.
The first 50% of the book is all slow burn, and the last 50% is almost straight mediocre spice. This wouldn't have been all bad if the grammar and spelling errors didn't start at the exact same time. Tongue is repeatedly misspelled in the middle of the spice.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 14, 2024
★★★★★ 5
Cozy Omegaverse
Format: Kindle
This is the true definition of Cozy Omegaverse. LA wedding coordinator meets her pack at the location for a couple’s destination wedding. Low angst because they are scent matched high heat with heats and knots. Everything you love about this genre with very little anxiety. Simply a fun experience to read and a book I will comeback to when I’m in a slump. I simply love reading this book
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 19, 2026
★★★★★ 4
Cute Omegaverse story
Format: Kindle
3.75 stars and 2 for spice. Adorable Omegaverse story about a wedding planner who has to go to a small town to plan a wedding for a movie star. While there, she meets her pack. This covers her struggles of not living there but trying to keep her relationship going with her 3 Alphas.
It's a quick read and cute, so I recommend it for people who like light-hearted RH stories.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 31, 2025
★★★★★ 3
real good
Format: Kindle
This was a solid read for the ABO universe. The characters are great. The smut scenes were well written. And the town is so cute I want to go there. It didn’t have much character development it just felt like I was getting to the characters as they were getting to know each other.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 27, 2025
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